1. Red-Top Tube (Clot Activator)
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- Additive: Clot activator or no additive.
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- Purpose: Used for serum determinations in chemistry tests. It helps the blood to clot, and the serum is separated by centrifugation.
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- Common Uses: Routine blood tests like cholesterol, liver function tests, and hormone assays.
2. Blue-Top Tube (Sodium Citrate)
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- Additive: Sodium citrate.
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- Purpose: Designed for coagulation studies by preventing blood from clotting immediately. The sodium citrate preserves clotting factors.
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- Common Uses: Tests like prothrombin time (PT) and activated partial thromboplastin time (aPTT) are commonly performed using this tube.
3. Purple-Top Tube (EDTA)
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- Additive: EDTA (Ethylenediaminetetraacetic acid).
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- Purpose: EDTA works by binding calcium ions, preventing blood clotting, and preserving cellular components. It’s used primarily for hematology tests.
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- Common Uses: Complete Blood Count (CBC), blood smears, and blood typing.
4. Green-Top Tube (Heparin)
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- Additive: Heparin.
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- Purpose: Heparin inhibits thrombin, thus preventing clot formation. It is commonly used for plasma determinations in chemistry.
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- Common Uses: Electrolyte panels, liver function tests, and some genetic testing.
5. Yellow-Top Tube (Serum Separator Tube – SST)
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- Additive: Clot activator and gel for serum separation.
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- Purpose: This tube contains a gel that separates the blood cells from the serum upon centrifugation, facilitating easy serum extraction.
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- Common Uses: Immunology, serology, and viral marker testing.
Why Color Coding Matters
The color-coded caps are not just for easy identification; they play a critical role in ensuring that the right type of test is conducted on the correct blood sample. Using the wrong tube could lead to inaccurate results, affecting diagnoses and patient care.
Conclusion
Whether you’re a phlebotomist, lab technician, or healthcare provider, understanding the different types of blood collection tubes is essential to performing accurate and reliable lab tests. Each tube serves a distinct purpose, and choosing the right one helps ensure the validity of lab results and overall patient safety.